Non-symbiotic germination propagation method for paphiopedilum strenuoides seeds

The invention discloses a non-symbiotic germination and propagation method for paphiopedilum fluviatilis seeds, and belongs to the technical field of plant tissue culture. The method comprises the following steps: harvesting capsules of paphiopedilum flutatum, performing surface disinfection, performing germination culture, differentiation culture, subculture multiplication culture and seedling strengthening and rooting culture on the seeds in sequence, and controlling the illumination condition of each culture stage. Specific maturity seeds of paphiopedilum fluminatum are used as explants, and complete plants of paphiopedilum fluminatum are obtained through germination, differentiation, subculture multiplication and seedling strengthening and rooting culture under specific culture medium and light environment conditions. The method is high in germination rate, rapid in seedling emergence and good in seedling quality, and a large number of high-quality paphiopedilum strenuoides seedlings can be produced withi