Improved size exclusion chromatography using low concentrations of amino acids in size exclusion chromatography mobile phase

The present disclosure relates to methods for performing size exclusion chromatography. Embodiments of the present disclosure feature methods for improving the separation of proteinaceous analytes in size exclusion chromatography, for example, by using low concentrations of amino acids or derivatives thereof in the mobile phase and using a stationary phase comprising porous particles surface-modified with hydroxyl-terminated polyethylene groups. 本公开涉及用于执行尺寸排阻色谱法的方法。本公开的实施方案的特征在于用于改善尺寸排阻色谱法中蛋白质分析物的分离的方法，例如，通过在流动相中使用低浓度的氨基酸或其衍生物以及使用包含用羟基封端的聚乙烯基团表面改性的多孔颗粒的固定相。