Flow detection method of neural stem cell marker

The invention discloses a flow type detection method of a neural stem cell marker. The flow type detection method comprises the following steps: (1) washing a sample; (2) receptor blocking: adding an Fc receptor blocking agent, and incubating; (3) fixing the cells: adding a Fixation Buffer fixing agent into the cell suspension, and incubating; (4) cell membrane rupture: adding a membrane rupture solution Perm Buffer III, and incubating; (5) cell staining: adding a corresponding antibody, and incubating; the antibodies are a Netin antibody, a Sox1 antibody and a Sox2 antibody; and (6) data analysis: performing flow detection, and analyzing expressions of Netin + and Sox1 + Sox2. According to the flow type detection method of the neural stem cell marker, the processes of receptor blocking, fixing, membrane rupture conditions and the like are optimized, the repeatability is high, the stability is good, and the problem that flow type detection is unstable in the process of process research and development is solv