Kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) with flow measurement chromatography immune test strip
The invention discloses a kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) amplification with a flow measurement chromatography immune test strip. The kit comprises a primer and a probe for detecting the African swine fever virus, the primer...
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| creator | TIAN HONG SHI ZHENGWANG JEONG HAE-HAK ZHANG XIAOYANG LUO JUNCONG ZHOU GAIJING ZHOU JING LIAO HUANCHEN ZHANG CHENGQI WAN YING |
| description | The invention discloses a kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) amplification with a flow measurement chromatography immune test strip. The kit comprises a primer and a probe for detecting the African swine fever virus, the primer is composed of an upstream primer and a downstream primer, the kit further comprises an amplification mixed enzyme for detecting the African swine fever virus, and the mixed enzyme contains a UNG enzyme. Compared with the prior art, the invention provides a rapid, simple, convenient and accurate detection reagent for molecular diagnosis of ASFV, and particularly has the advantages of low requirements on instruments and equipment, only need of a constant-temperature water bath kettle or a constant-temperature device, high detection speed (about 30 minutes), and is not only suitable for quantitative analysis of scientific research institutions, but also suitable for prevention and control units at all levels and gra |
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The kit comprises a primer and a probe for detecting the African swine fever virus, the primer is composed of an upstream primer and a downstream primer, the kit further comprises an amplification mixed enzyme for detecting the African swine fever virus, and the mixed enzyme contains a UNG enzyme. Compared with the prior art, the invention provides a rapid, simple, convenient and accurate detection reagent for molecular diagnosis of ASFV, and particularly has the advantages of low requirements on instruments and equipment, only need of a constant-temperature water bath kettle or a constant-temperature device, high detection speed (about 30 minutes), and is not only suitable for quantitative analysis of scientific research institutions, but also suitable for prevention and control units at all levels and gra</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230502&DB=EPODOC&CC=CN&NR=116042908A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230502&DB=EPODOC&CC=CN&NR=116042908A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TIAN HONG</creatorcontrib><creatorcontrib>SHI ZHENGWANG</creatorcontrib><creatorcontrib>JEONG HAE-HAK</creatorcontrib><creatorcontrib>ZHANG XIAOYANG</creatorcontrib><creatorcontrib>LUO JUNCONG</creatorcontrib><creatorcontrib>ZHOU GAIJING</creatorcontrib><creatorcontrib>ZHOU JING</creatorcontrib><creatorcontrib>LIAO HUANCHEN</creatorcontrib><creatorcontrib>ZHANG CHENGQI</creatorcontrib><creatorcontrib>WAN YING</creatorcontrib><title>Kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) with flow measurement chromatography immune test strip</title><description>The invention discloses a kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) amplification with a flow measurement chromatography immune test strip. The kit comprises a primer and a probe for detecting the African swine fever virus, the primer is composed of an upstream primer and a downstream primer, the kit further comprises an amplification mixed enzyme for detecting the African swine fever virus, and the mixed enzyme contains a UNG enzyme. 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The kit comprises a primer and a probe for detecting the African swine fever virus, the primer is composed of an upstream primer and a downstream primer, the kit further comprises an amplification mixed enzyme for detecting the African swine fever virus, and the mixed enzyme contains a UNG enzyme. Compared with the prior art, the invention provides a rapid, simple, convenient and accurate detection reagent for molecular diagnosis of ASFV, and particularly has the advantages of low requirements on instruments and equipment, only need of a constant-temperature water bath kettle or a constant-temperature device, high detection speed (about 30 minutes), and is not only suitable for quantitative analysis of scientific research institutions, but also suitable for prevention and control units at all levels and gra</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) with flow measurement chromatography immune test strip |
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