Kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) with flow measurement chromatography immune test strip
The invention discloses a kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) amplification with a flow measurement chromatography immune test strip. The kit comprises a primer and a probe for detecting the African swine fever virus, the primer...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a kit for rapidly detecting African swine fever virus by combining RPA (recombinase polymerase amplification) amplification with a flow measurement chromatography immune test strip. The kit comprises a primer and a probe for detecting the African swine fever virus, the primer is composed of an upstream primer and a downstream primer, the kit further comprises an amplification mixed enzyme for detecting the African swine fever virus, and the mixed enzyme contains a UNG enzyme. Compared with the prior art, the invention provides a rapid, simple, convenient and accurate detection reagent for molecular diagnosis of ASFV, and particularly has the advantages of low requirements on instruments and equipment, only need of a constant-temperature water bath kettle or a constant-temperature device, high detection speed (about 30 minutes), and is not only suitable for quantitative analysis of scientific research institutions, but also suitable for prevention and control units at all levels and gra |
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