Method for establishing senescent cell model by reducing NAD < + >/NADH level by interfering Slc12a8

The invention relates to a novel method for inducing establishment of an aging cell model by interference of Slc12a8 to cause reduction of NAD < + >/NADH level, and the method comprises the following steps: interfering a cell Slc12a8 gene for 48 hours by using small interfering RNA to obtain the aging cell model. The model constructed by the method has the advantages that the modeling time is shortened, toxic damage, oxidative stress and PARP1 and SIRT1 protein down-regulation can be caused at the same time, and experimental conditions are easy to control. According to the model, small interfering RNA is dissolved by sterilized ultrapure water in vitro, so that the concentration of a working solution is 0.1 mmol. L , the working solution can be directly added into NCM460, HT29 and HCT116 cells, and the model is evaluated by detecting cytotoxicity, NAD < + >/NADH level and cell oxidative stress change. 本发明涉及通过干扰Slc12a8导致NAD+/NADH水平降低诱导建立衰老细胞模型的新方法，步骤如下：使用小干扰RNA干扰细胞Slc12a8基因48h后，即得衰老细胞模型。本发明方法构建的模型的优势在于缩短建