Colloidal gold immunochromatography kit and manufacturing method thereof

The invention discloses a colloidal gold immunochromatography kit which comprises a bottom plate, and a water absorption pad, a nitrocellulose membrane, a gold pad and a sample pad which are sequentially arranged on the bottom plate, the nitrocellulose membrane is coated with a plurality of quality...

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Bibliographische Detailangaben
Hauptverfasser: MA HONGYAN, GUO GUILING, LIANG HUANKUN, CHEN CUICUI, LAI HONGRUI, LI LAIQING, ZHONG SHUHAI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a colloidal gold immunochromatography kit which comprises a bottom plate, and a water absorption pad, a nitrocellulose membrane, a gold pad and a sample pad which are sequentially arranged on the bottom plate, the nitrocellulose membrane is coated with a plurality of quality control lines and detection lines, the detection lines are respectively coated with anti-MMP9, LP-PLA2, hs-CRP and Lp (a) paired monoclonal antibodies, the quality control lines are coated with goat anti-mouse IgG secondary antibodies, and the detection lines are coated with goat anti-mouse IgG secondary antibodies. The number of the gold pads is more than one, and the gold pads are respectively impregnated with an anti-MMP9, LP-PLA2, hs-CRP or Lp (a) antibody-colloidal gold compound; the colloidal gold immunochromatography kit and the manufacturing method thereof can be simultaneously used for detecting four types of human atherosclerotic plaque stability markers, and four types of high-specificity biomarkers incl