Antibody-fluorescent nano-microsphere conjugate for synchronously detecting antibiotics enrofloxacin and ciprofloxacin and preparation method of antibody-fluorescent nano-microsphere conjugate

The preparation method comprises the following steps: carrying out carboxylation modification on the surface of a polystyrene microsphere in a hydrolysis manner, and then preparing the antibody-fluorescent nano-microsphere conjugate by utilizing an embedding manner, so as to obtain the antibody-fluo...

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Hauptverfasser:	WANG HONGLIAN, YANG TINGTING, WANG XINCHI, SONG CHAO, KUANG QUN, MENG SHUNLONG, QI SUXIAN, QIU LIPING, ZHANG HAITAO, FAN LIMIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING PHYSICS TESTING
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creator	WANG HONGLIAN YANG TINGTING WANG XINCHI SONG CHAO KUANG QUN MENG SHUNLONG QI SUXIAN QIU LIPING ZHANG HAITAO FAN LIMIN
description	The preparation method comprises the following steps: carrying out carboxylation modification on the surface of a polystyrene microsphere in a hydrolysis manner, and then preparing the antibody-fluorescent nano-microsphere conjugate by utilizing an embedding manner, so as to obtain the antibody-fluorescent nano-microsphere conjugate for synchronously detecting the antibiotic enrofloxacin and the antibiotic ciprofloxacin. Embedding a rare earth europium element in the microspheres to form a complex of the polystyrene microspheres and the europium element to obtain fluorescent microspheres; constructing a pretreatment system of the fluorescent microspheres, and cleaning and activating the fluorescent microspheres; the fluorescent microspheres are coupled with an anti-enrofloxacin or anti-ciprofloxacin monoclonal antibody; the prepared product is stored in a dark place at 2-8 DEG C. The conjugate prepared by using the method disclosed by the invention not only can greatly improve the detection sensitivity of the
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Embedding a rare earth europium element in the microspheres to form a complex of the polystyrene microspheres and the europium element to obtain fluorescent microspheres; constructing a pretreatment system of the fluorescent microspheres, and cleaning and activating the fluorescent microspheres; the fluorescent microspheres are coupled with an anti-enrofloxacin or anti-ciprofloxacin monoclonal antibody; the prepared product is stored in a dark place at 2-8 DEG C. 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title	Antibody-fluorescent nano-microsphere conjugate for synchronously detecting antibiotics enrofloxacin and ciprofloxacin and preparation method of antibody-fluorescent nano-microsphere conjugate
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