Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer
The invention relates to nested PCR (polymerase chain reaction) amplification primers for detecting duck hepatitis C virus and application of the nested PCR amplification primers, and relates to the technical field of virus detection. The invention relates to a nested PCR (Polymerase Chain Reaction)...
Gespeichert in:
| Hauptverfasser: | , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | HUANG SHUJIAN LYU ZHIHANG YAO XINYAN ZHANG XUELIAN ZHANG YUQIAN SHAO JIANWEI |
| description | The invention relates to nested PCR (polymerase chain reaction) amplification primers for detecting duck hepatitis C virus and application of the nested PCR amplification primers, and relates to the technical field of virus detection. The invention relates to a nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus. The nested PCR amplification primer comprises the following primers: DHCV-F1: ACTTCCACCAACGAAGT (SEQ ID NO: 1); dHCV-F2: TGCTGTCGGTGCGTCAAT (SEQ ID NO: 2), and DHCV-F2: TGCTGTCGGTGCGTCAAT And DHCV-R: AGCACACGTTCTTACAGCAGA (SEQ ID NO: 3), and DHCV-R: When the nested PCR amplification primer is used for detecting the duck hepatitis C virus, the nested PCR amplification primer has very high sensitivity and very strong specificity, the detection cost and the detection time are greatly reduced, and the nested PCR amplification primer can be used for clinical sample detection and large-scale epidemiological investigation.
本发明涉及一种用于检测鸭丙型肝炎病毒的巢式PCR扩增引物及其应用，涉及病毒检测技 |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN116024380A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN116024380A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN116024380A3</originalsourceid><addsrcrecordid>eNqNjbEKwjAURbs4iPoPz00HobUirhIUp1KKe3kkLzaYJiFJBb_DHzZCwcXB6XLhnHun2auiEElAzRpY1VY_e_IYCFiHykBDyKOyZg3YO62k4vip4LxKHEjrQVCkxJgbiIHfoSOXkKgCMHgoPwRAIwBdskfXSjDfz1-782wiUQdajDnLlufTlV025GxLwSEnQ7FlVVHs8-2uPOTH8h_mDc7MT2w</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer</title><source>esp@cenet</source><creator>HUANG SHUJIAN ; LYU ZHIHANG ; YAO XINYAN ; ZHANG XUELIAN ; ZHANG YUQIAN ; SHAO JIANWEI</creator><creatorcontrib>HUANG SHUJIAN ; LYU ZHIHANG ; YAO XINYAN ; ZHANG XUELIAN ; ZHANG YUQIAN ; SHAO JIANWEI</creatorcontrib><description>The invention relates to nested PCR (polymerase chain reaction) amplification primers for detecting duck hepatitis C virus and application of the nested PCR amplification primers, and relates to the technical field of virus detection. The invention relates to a nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus. The nested PCR amplification primer comprises the following primers: DHCV-F1: ACTTCCACCAACGAAGT (SEQ ID NO: 1); dHCV-F2: TGCTGTCGGTGCGTCAAT (SEQ ID NO: 2), and DHCV-F2: TGCTGTCGGTGCGTCAAT And DHCV-R: AGCACACGTTCTTACAGCAGA (SEQ ID NO: 3), and DHCV-R: When the nested PCR amplification primer is used for detecting the duck hepatitis C virus, the nested PCR amplification primer has very high sensitivity and very strong specificity, the detection cost and the detection time are greatly reduced, and the nested PCR amplification primer can be used for clinical sample detection and large-scale epidemiological investigation.
本发明涉及一种用于检测鸭丙型肝炎病毒的巢式PCR扩增引物及其应用，涉及病毒检测技</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230428&DB=EPODOC&CC=CN&NR=116024380A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25543,76293</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230428&DB=EPODOC&CC=CN&NR=116024380A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>HUANG SHUJIAN</creatorcontrib><creatorcontrib>LYU ZHIHANG</creatorcontrib><creatorcontrib>YAO XINYAN</creatorcontrib><creatorcontrib>ZHANG XUELIAN</creatorcontrib><creatorcontrib>ZHANG YUQIAN</creatorcontrib><creatorcontrib>SHAO JIANWEI</creatorcontrib><title>Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer</title><description>The invention relates to nested PCR (polymerase chain reaction) amplification primers for detecting duck hepatitis C virus and application of the nested PCR amplification primers, and relates to the technical field of virus detection. The invention relates to a nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus. The nested PCR amplification primer comprises the following primers: DHCV-F1: ACTTCCACCAACGAAGT (SEQ ID NO: 1); dHCV-F2: TGCTGTCGGTGCGTCAAT (SEQ ID NO: 2), and DHCV-F2: TGCTGTCGGTGCGTCAAT And DHCV-R: AGCACACGTTCTTACAGCAGA (SEQ ID NO: 3), and DHCV-R: When the nested PCR amplification primer is used for detecting the duck hepatitis C virus, the nested PCR amplification primer has very high sensitivity and very strong specificity, the detection cost and the detection time are greatly reduced, and the nested PCR amplification primer can be used for clinical sample detection and large-scale epidemiological investigation.
本发明涉及一种用于检测鸭丙型肝炎病毒的巢式PCR扩增引物及其应用，涉及病毒检测技</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNjbEKwjAURbs4iPoPz00HobUirhIUp1KKe3kkLzaYJiFJBb_DHzZCwcXB6XLhnHun2auiEElAzRpY1VY_e_IYCFiHykBDyKOyZg3YO62k4vip4LxKHEjrQVCkxJgbiIHfoSOXkKgCMHgoPwRAIwBdskfXSjDfz1-782wiUQdajDnLlufTlV025GxLwSEnQ7FlVVHs8-2uPOTH8h_mDc7MT2w</recordid><startdate>20230428</startdate><enddate>20230428</enddate><creator>HUANG SHUJIAN</creator><creator>LYU ZHIHANG</creator><creator>YAO XINYAN</creator><creator>ZHANG XUELIAN</creator><creator>ZHANG YUQIAN</creator><creator>SHAO JIANWEI</creator><scope>EVB</scope></search><sort><creationdate>20230428</creationdate><title>Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer</title><author>HUANG SHUJIAN ; LYU ZHIHANG ; YAO XINYAN ; ZHANG XUELIAN ; ZHANG YUQIAN ; SHAO JIANWEI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116024380A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>HUANG SHUJIAN</creatorcontrib><creatorcontrib>LYU ZHIHANG</creatorcontrib><creatorcontrib>YAO XINYAN</creatorcontrib><creatorcontrib>ZHANG XUELIAN</creatorcontrib><creatorcontrib>ZHANG YUQIAN</creatorcontrib><creatorcontrib>SHAO JIANWEI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>HUANG SHUJIAN</au><au>LYU ZHIHANG</au><au>YAO XINYAN</au><au>ZHANG XUELIAN</au><au>ZHANG YUQIAN</au><au>SHAO JIANWEI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer</title><date>2023-04-28</date><risdate>2023</risdate><abstract>The invention relates to nested PCR (polymerase chain reaction) amplification primers for detecting duck hepatitis C virus and application of the nested PCR amplification primers, and relates to the technical field of virus detection. The invention relates to a nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus. The nested PCR amplification primer comprises the following primers: DHCV-F1: ACTTCCACCAACGAAGT (SEQ ID NO: 1); dHCV-F2: TGCTGTCGGTGCGTCAAT (SEQ ID NO: 2), and DHCV-F2: TGCTGTCGGTGCGTCAAT And DHCV-R: AGCACACGTTCTTACAGCAGA (SEQ ID NO: 3), and DHCV-R: When the nested PCR amplification primer is used for detecting the duck hepatitis C virus, the nested PCR amplification primer has very high sensitivity and very strong specificity, the detection cost and the detection time are greatly reduced, and the nested PCR amplification primer can be used for clinical sample detection and large-scale epidemiological investigation.
本发明涉及一种用于检测鸭丙型肝炎病毒的巢式PCR扩增引物及其应用，涉及病毒检测技</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN116024380A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T14%3A47%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=HUANG%20SHUJIAN&rft.date=2023-04-28&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN116024380A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |