Nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus and application of nested PCR amplification primer

The invention relates to nested PCR (polymerase chain reaction) amplification primers for detecting duck hepatitis C virus and application of the nested PCR amplification primers, and relates to the technical field of virus detection. The invention relates to a nested PCR (Polymerase Chain Reaction) amplification primer for detecting duck hepatitis C virus. The nested PCR amplification primer comprises the following primers: DHCV-F1: ACTTCCACCAACGAAGT (SEQ ID NO: 1); dHCV-F2: TGCTGTCGGTGCGTCAAT (SEQ ID NO: 2), and DHCV-F2: TGCTGTCGGTGCGTCAAT And DHCV-R: AGCACACGTTCTTACAGCAGA (SEQ ID NO: 3), and DHCV-R: When the nested PCR amplification primer is used for detecting the duck hepatitis C virus, the nested PCR amplification primer has very high sensitivity and very strong specificity, the detection cost and the detection time are greatly reduced, and the nested PCR amplification primer can be used for clinical sample detection and large-scale epidemiological investigation. 本发明涉及一种用于检测鸭丙型肝炎病毒的巢式PCR扩增引物及其应用，涉及病毒检测技