Specific primer pair and application thereof in identifying whitmania pigra
The invention relates to the technical field of whitmania pigra identification, in particular to a specific primer pair and application of the specific primer pair in whitmania pigra identification, the primer pair comprises a primer pair KT1-3 or a primer pair KT1-4, when the primer pair and an ide...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to the technical field of whitmania pigra identification, in particular to a specific primer pair and application of the specific primer pair in whitmania pigra identification, the primer pair comprises a primer pair KT1-3 or a primer pair KT1-4, when the primer pair and an identification method provided by the invention are adopted for an identification test, the primer pair is not influenced by sample character integrity, the operation is simple and easy to implement, and the identification efficiency is high. Whether a sample is whitmania pigra or contains whitmania pigra components can be judged only by extracting the genome DNA of the sample, adopting the primer pair KT1-3 or the primer pair KT1-4 for PCR reaction and detecting the strip size of a PCR product through agarose gel electrophoresis, and non-specific reaction is not generated on other leech varieties, so that the detection is simple, convenient and accurate.
本发明涉及宽体金线蛭鉴别技术领域,具体涉及一种特异性引物对及其在鉴别宽体金线蛭中的应用,引物对包括引物对KT1-3或引物对KT |
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