Rhodosporidium toruloides engineering strain with high nervonic acid yield as well as construction method and application thereof

The invention discloses a high-nervonic-acid-yield Rhodosporidium toruloides engineering strain as well as a construction method and application thereof, the engineering strain takes Rhodosporidium toruloides as an original strain, and performs heterologous expression on KCS enzyme in fatty acid elongase compounds with different substrate preferences; kCR, HCD and ECR enzymes and malic enzyme ME1 in the fatty acid elongase complex are overexpressed. According to the high-yield nervonic acid strain constructed by the invention, the titers of grease and nervonic acid respectively reach 73.7 g/L and 35.4 g/L at the level of a 50L fermentation tank, so that the strain has potential industrial production value. 本发明公开了一种高产神经酸的圆红冬孢酵母工程菌株及其构建方法和应用，该工程菌株以圆红冬孢酵母为出发菌株，异源表达不同底物偏好性的脂肪酸延伸酶复合物中的KCS酶；过表达脂肪酸延伸酶复合物中的KCR、HCD和ECR酶，以及苹果酸酶ME1。本发明构建的高产神经酸的菌株，在50L发酵罐水平上，油脂和神经酸滴度分别达到73.7g/L和35.4g/L，具有潜在的工业化生产价值。