Targeted fully humanized CD70 chimeric antigen receptor and application thereof

The invention belongs to the technical field of tumor cell immunotherapy, and particularly relates to a chimeric antigen receptor targeting fully humanized CD70 and application of the chimeric antigen receptor. The chimeric antigen receptor comprises an extracellular structural domain, a hinge region, a transmembrane region and an intracellular signal domain which are sequentially connected, wherein the extracellular structural domain comprises an amino acid sequence as shown in Sequence NO.1 or a functional variant of the amino acid sequence. The extracellular domain further comprises a CD8 signal peptide, and the CD8 signal peptide comprises an amino acid sequence as shown in Sequence NO.2 or a functional variant of the amino acid sequence as shown in Sequence NO.2. The chimeric antigen receptor and the CAR-T cell of the chimeric antigen receptor can effectively kill 786-O-Luc-GFP target cells, secrete an effective amount of cell factors, can effectively inhibit the growth of tumors in vivo, and have a rema