PCR (Polymerase Chain Reaction) detection primer and detection method for banana grey rot pathogen
The invention provides a PCR (Polymerase Chain Reaction) detection primer and a detection method of banana grey rot germs. The sequence of an upstream primer in the PCR detection primer is ATGTGCAAGGCCGGTTTCG, and the sequence of a downstream primer in the PCR detection primer is CGTCACATGGCTCAAATCG...
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| creator | YU QUNFANG QI YANXIANG ZHANG XIN PENG JUN XIE YIXIAN ZENG FANYUN |
| description | The invention provides a PCR (Polymerase Chain Reaction) detection primer and a detection method of banana grey rot germs. The sequence of an upstream primer in the PCR detection primer is ATGTGCAAGGCCGGTTTCG, and the sequence of a downstream primer in the PCR detection primer is CGTCACATGGCTCAAATCG. Based on the specific primer, the PCR detection method of the banana gray rot pathogen is developed. The primers and the detection method are used for rapidly detecting the banana grey rot germs, pathogens can be distinguished by combining one-time PCR reaction with sequencing analysis, the defects of a conventional identification method are overcome, and the primers and the detection method have the advantages of being high in specificity, accuracy and sensitivity, wide in applicability, easy and convenient to operate and the like.
本发明提供一种香蕉灰纹病菌的PCR检测引物及检测方法，所述PCR检测引物中的上游引物序列为ATGTGCAAGGCCGGTTTCG，下游引物序列为CGTCACATCGGCTCAAATCG。基于该特异性引物，开发出香蕉灰纹病菌的PCR检测方法。利用该引物和检测方法快速检测香蕉灰纹病菌，一次PCR反应结合测序分析即可区分病原物，克服常规鉴定方法的不足，具有特异性强，准确 |
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本发明提供一种香蕉灰纹病菌的PCR检测引物及检测方法，所述PCR检测引物中的上游引物序列为ATGTGCAAGGCCGGTTTCG，下游引物序列为CGTCACATCGGCTCAAATCG。基于该特异性引物，开发出香蕉灰纹病菌的PCR检测方法。利用该引物和检测方法快速检测香蕉灰纹病菌，一次PCR反应结合测序分析即可区分病原物，克服常规鉴定方法的不足，具有特异性强，准确</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230425&DB=EPODOC&CC=CN&NR=116004892A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230425&DB=EPODOC&CC=CN&NR=116004892A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>YU QUNFANG</creatorcontrib><creatorcontrib>QI YANXIANG</creatorcontrib><creatorcontrib>ZHANG XIN</creatorcontrib><creatorcontrib>PENG JUN</creatorcontrib><creatorcontrib>XIE YIXIAN</creatorcontrib><creatorcontrib>ZENG FANYUN</creatorcontrib><title>PCR (Polymerase Chain Reaction) detection primer and detection method for banana grey rot pathogen</title><description>The invention provides a PCR (Polymerase Chain Reaction) detection primer and a detection method of banana grey rot germs. The sequence of an upstream primer in the PCR detection primer is ATGTGCAAGGCCGGTTTCG, and the sequence of a downstream primer in the PCR detection primer is CGTCACATGGCTCAAATCG. Based on the specific primer, the PCR detection method of the banana gray rot pathogen is developed. The primers and the detection method are used for rapidly detecting the banana grey rot germs, pathogens can be distinguished by combining one-time PCR reaction with sequencing analysis, the defects of a conventional identification method are overcome, and the primers and the detection method have the advantages of being high in specificity, accuracy and sensitivity, wide in applicability, easy and convenient to operate and the like.
本发明提供一种香蕉灰纹病菌的PCR检测引物及检测方法，所述PCR检测引物中的上游引物序列为ATGTGCAAGGCCGGTTTCG，下游引物序列为CGTCACATCGGCTCAAATCG。基于该特异性引物，开发出香蕉灰纹病菌的PCR检测方法。利用该引物和检测方法快速检测香蕉灰纹病菌，一次PCR反应结合测序分析即可区分病原物，克服常规鉴定方法的不足，具有特异性强，准确</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNirEKwjAYhLM4iPoOv5sOQqsiOkpQnKQU9_K3ubaBNglJlr69QRwc5YY7vvvmoi5kSZvCDtMIzwEke9aGSnATtTVbUoj4THJeJ4fYqB84IvZWUWs91WxSqPOYyNtIjtPVwSzFrOUhYPXthVjfby_52MHZCsFxA4NYyWeen7LseL7sr4d_nDewbD0h</recordid><startdate>20230425</startdate><enddate>20230425</enddate><creator>YU QUNFANG</creator><creator>QI YANXIANG</creator><creator>ZHANG XIN</creator><creator>PENG JUN</creator><creator>XIE YIXIAN</creator><creator>ZENG FANYUN</creator><scope>EVB</scope></search><sort><creationdate>20230425</creationdate><title>PCR (Polymerase Chain Reaction) detection primer and detection method for banana grey rot pathogen</title><author>YU QUNFANG ; QI YANXIANG ; ZHANG XIN ; PENG JUN ; XIE YIXIAN ; ZENG FANYUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN116004892A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>YU QUNFANG</creatorcontrib><creatorcontrib>QI YANXIANG</creatorcontrib><creatorcontrib>ZHANG XIN</creatorcontrib><creatorcontrib>PENG JUN</creatorcontrib><creatorcontrib>XIE YIXIAN</creatorcontrib><creatorcontrib>ZENG FANYUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>YU QUNFANG</au><au>QI YANXIANG</au><au>ZHANG XIN</au><au>PENG JUN</au><au>XIE YIXIAN</au><au>ZENG FANYUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>PCR (Polymerase Chain Reaction) detection primer and detection method for banana grey rot pathogen</title><date>2023-04-25</date><risdate>2023</risdate><abstract>The invention provides a PCR (Polymerase Chain Reaction) detection primer and a detection method of banana grey rot germs. The sequence of an upstream primer in the PCR detection primer is ATGTGCAAGGCCGGTTTCG, and the sequence of a downstream primer in the PCR detection primer is CGTCACATGGCTCAAATCG. Based on the specific primer, the PCR detection method of the banana gray rot pathogen is developed. The primers and the detection method are used for rapidly detecting the banana grey rot germs, pathogens can be distinguished by combining one-time PCR reaction with sequencing analysis, the defects of a conventional identification method are overcome, and the primers and the detection method have the advantages of being high in specificity, accuracy and sensitivity, wide in applicability, easy and convenient to operate and the like.
本发明提供一种香蕉灰纹病菌的PCR检测引物及检测方法，所述PCR检测引物中的上游引物序列为ATGTGCAAGGCCGGTTTCG，下游引物序列为CGTCACATCGGCTCAAATCG。基于该特异性引物，开发出香蕉灰纹病菌的PCR检测方法。利用该引物和检测方法快速检测香蕉灰纹病菌，一次PCR反应结合测序分析即可区分病原物，克服常规鉴定方法的不足，具有特异性强，准确</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | PCR (Polymerase Chain Reaction) detection primer and detection method for banana grey rot pathogen |
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