PCR (Polymerase Chain Reaction) detection primer and detection method for banana grey rot pathogen

The invention provides a PCR (Polymerase Chain Reaction) detection primer and a detection method of banana grey rot germs. The sequence of an upstream primer in the PCR detection primer is ATGTGCAAGGCCGGTTTCG, and the sequence of a downstream primer in the PCR detection primer is CGTCACATGGCTCAAATCG. Based on the specific primer, the PCR detection method of the banana gray rot pathogen is developed. The primers and the detection method are used for rapidly detecting the banana grey rot germs, pathogens can be distinguished by combining one-time PCR reaction with sequencing analysis, the defects of a conventional identification method are overcome, and the primers and the detection method have the advantages of being high in specificity, accuracy and sensitivity, wide in applicability, easy and convenient to operate and the like. 本发明提供一种香蕉灰纹病菌的PCR检测引物及检测方法，所述PCR检测引物中的上游引物序列为ATGTGCAAGGCCGGTTTCG，下游引物序列为CGTCACATCGGCTCAAATCG。基于该特异性引物，开发出香蕉灰纹病菌的PCR检测方法。利用该引物和检测方法快速检测香蕉灰纹病菌，一次PCR反应结合测序分析即可区分病原物，克服常规鉴定方法的不足，具有特异性强，准确