Specific primer of molecular marker of Mongolian sheep gene LEPR and application of specific primer

The invention discloses an LEPR gene molecular marker and a use method thereof, and belongs to the field of molecular biology. The method comprises the following steps: detecting whether T-C mutation exists in a non-coding region 43174682bp of a Mongolian sheep genome LEPR gene or not, determining t...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	CHAO LUMENG, WANG LIANHU, TONG BIN, LI NA, CHENG ZIXUAN, ZHANG XIUYING, YU HAIQUAN, ZHU YONG, AO DENGGE RILE, YANG MINGTAO, CAO ZIWEI, HE MEI, CHE-LI MUGE, CHANG ZHU, SHUANG SHAN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The invention discloses an LEPR gene molecular marker and a use method thereof, and belongs to the field of molecular biology. The method comprises the following steps: detecting whether T-C mutation exists in a non-coding region 43174682bp of a Mongolian sheep genome LEPR gene or not, determining the genotype of a Mongolian sheep individual at the site, screening breeding Mongolian sheep by utilizing the genotype, and carrying out single nucleotide polymorphism detection on the LEPR gene by adopting a DNA (Deoxyribonucleic Acid) sequencing technology so as to compare the polymorphism of the LEPR gene in a Mongolian sheep variety. And then carrying out sequencing comparative analysis on PCR product fragments to find out the molecular marker related to reproductive capacity. The molecular marker polymorphism is rapidly, simply and conveniently detected by using DNA sequencing, and an accurate, simple and convenient detection method is provided for increasing the lambing number of Mongolian sheep by using a mol