Primer for multiple STR typing of zaocys dhumnade medicinal materials, standard decoction and traditional Chinese medicine formula granules as well as application and identification method of primer

The invention discloses primers for multiple STR typing of zaocys dhumnade medicinal materials, standard decoction and traditional Chinese medicine formula granules. The primers comprise a first primer pair, a second primer pair, a third primer pair and a fourth primer pair, the sequences of the primers are as shown in SEQ ID NO: 1-8. The invention further discloses application of the primer, a kit based on the primer and an identification method based on the primer. By implementing the method, zaocys dhumnade, elaphe carinata, ptyas korros and elaphe mollis can be effectively identified at the same time. 本发明公开了一种用于乌梢蛇药材、标准汤剂及中药配方颗粒多重STR分型的引物，其包括第一引物对、第二引物对、第三引物对和第四引物对；其序列如SEQ ID NO：1～8所示。本发明还公开了上述引物的应用，基于该引物的试剂盒，以及基于该引物的鉴别方法。实施本发明，可同时有效鉴别乌梢蛇、王锦蛇、灰鼠蛇和百花锦蛇。