Method for preparing (-) gamma-lactam through conversion and resolution of (+) gamma-lactamase

The invention belongs to the technical field of bioengineering, and discloses a method for preparing (-) gamma-lactam through conversion and resolution of (+) gamma-lactamase. The method comprises the following steps: step 1, carrying out microbial fermentation to produce (+) gamma-lactamase; step 2...

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Bibliographische Detailangaben
Hauptverfasser: WANG CONG, LIU CHUNYING, CHI XUEMEI, XIA SHUIYING, GUO HONGZHI, CHI NAIYU, YU SHUANG, YUAN WENTAO, ZHANG QINGFANG, WANG YAN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the technical field of bioengineering, and discloses a method for preparing (-) gamma-lactam through conversion and resolution of (+) gamma-lactamase. The method comprises the following steps: step 1, carrying out microbial fermentation to produce (+) gamma-lactamase; step 2, preparing a crude enzyme liquid sample; step 3, enzyme liquid concentration; and step 4, producing (-) gamma-lactam. The method for producing the (-) gamma-lactam through conversion of the (+) gamma-lactamase is provided for the first time, and the method is low in production cost and short in production period; materials used for concentrating the enzyme liquid can be recycled, the method is environment-friendly, the operation process is simple, and the enzyme activity recovery rate is high; the method realizes efficient production of the (-) gamma-lactam with high medical value by using the low-value (+/-) gamma-lactam as the raw material, lays a solid clinical foundation for synthesis of (-) gamma-lactam drugs