Aflatoxin oxidase with improved pepsin resistance

The invention discloses aflatoxin oxidase (AFO) with improved pepsin resistance and application of the aflatoxin oxidase (AFO). According to the aflatoxin oxidase mutant, key amino acid residues in wild aflatoxin oxidase molecules are modified through a protein engineering technology, and the mutant...

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Hauptverfasser:	HUANG JIONGWEI, GUO MENGYING, MO SHIYI, QIAN DAN, LIU DALING, YAO DONGSHENG, LIU GUIZHEN, XIE CHUNFANG, LAO WEIMING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses aflatoxin oxidase (AFO) with improved pepsin resistance and application of the aflatoxin oxidase (AFO). According to the aflatoxin oxidase mutant, key amino acid residues in wild aflatoxin oxidase molecules are modified through a protein engineering technology, and the mutant aflatoxin oxidase with the pepsin resistance improved is obtained through screening. The resistance half-life period of the mutant aflatoxin oxidase (AFOG475M) acting on the aflatoxin (AFB1) to pepsin is 3.78 times that of wild type AFO (Aflatoxin Oxidase). Compared with a wild type, the mutant AFOG475M keeps more than 40% of activity at 10-40 DEG C; the optimal reaction pH is 7.5; and when the pH value is 5.0-8.0, the activity is kept by more than 40%. 本发明公开了一种对胃蛋白酶抗性提高的黄曲霉毒素氧化酶(AFO)及其应用。本发明通过蛋白质工程技术对野生型黄曲霉毒素氧化酶分子内的关键氨基酸残基进行了改造，筛选得到了一种对胃蛋白酶抗性提高的突变体黄曲霉毒素氧化酶。本发明所述的作用于黄曲霉毒素(AFB1)的突变体黄曲霉毒素氧化酶(AFOG475M)对胃蛋白酶的抗性半衰期是野生型AFO的3.78倍。相比于野生型，突变体AFOG475M在10～40℃时保有40％以上的活性；最适反应pH为7.5；在pH5.0～8.0时保有40％以上的活性。