Preparation method of multi-protein mixture for promoting formation of human bones

The invention discloses a preparation method of a multi-protein mixture for promoting human bone formation, which comprises the following steps: performing ultrafiltration on a protein dissolving solution through a semi-permeable membrane, taking an overfiltrate as collagen and an underfiltrate as b...

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Hauptverfasser: TANG RUICHEN, LI XIANGJIE, DING LI, REN FANG, GUO CHANGSHUI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a preparation method of a multi-protein mixture for promoting human bone formation, which comprises the following steps: performing ultrafiltration on a protein dissolving solution through a semi-permeable membrane, taking an overfiltrate as collagen and an underfiltrate as bone morphogenetic protein, mixing the obtained collagen and bone morphogenetic protein, and performing composite freeze-drying and subsequent sterilization treatment on the mixture, thereby obtaining the multi-protein mixture for promoting human bone formation. An optical picture obtained by a mixture activity detection experiment shows that a large number of active cartilage cells can be seen by naked eyes, which proves that the prepared multi-protein mixture has an induced generation effect on the cartilage cells, and shows that the bone morphogenetic proteins are stored by using a method for protecting the bone morphogenetic proteins by using collagen; the prepared mixture can effectively relieve the activity lo