Method for preparing oligonucleotides using improved oxidation scheme

The present invention relates to a process for the production of mixed P = O/P = S backbone oligonucleotides comprising the use of a novel oxidation protocol and a novel oxidation solution, according to which the intermediate phosphite triester compound of formula I is selectively oxidized to the phosphate diester compound of formula II. 本发明涉及一种用于产生混合的P＝O/P＝S主链寡核苷酸的方法，所述方法包括应用新型氧化方案和新颖氧化溶液，根据方案将式I的中间体亚磷酸三酯化合物选择性地氧化成式II的磷酸二酯化合物。