Zearalenone detoxification enzyme ZLHY-6 with relatively high thermal stability

The invention discloses a zearalenone detoxification enzyme ZLHY-6 with high thermal stability, the zearalenone detoxification enzyme ZLHY-6 comprises a mutant H134W or a mutant Q45C/A253C, an amino acid sequence for coding the mutant H134W is as shown in SEQ ID NO.1, and an amino acid sequence for...

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Hauptverfasser:	DU HAOLAN, JIN NUO, XING FUGUO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FODDER FOODS OR FOODSTUFFS FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BYSUBCLASSES A23B - A23J HUMAN NECESSITIES METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OFNUTRITIVE QUALITIES, PHYSICAL TREATMENT THEIR TREATMENT, NOT COVERED BY OTHER CLASSES VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a zearalenone detoxification enzyme ZLHY-6 with high thermal stability, the zearalenone detoxification enzyme ZLHY-6 comprises a mutant H134W or a mutant Q45C/A253C, an amino acid sequence for coding the mutant H134W is as shown in SEQ ID NO.1, and an amino acid sequence for coding the mutant Q45C/A253C is as shown in SEQ ID NO.2. The zearalenone detoxification enzyme ZLHY-6 is a zearalenone detoxification enzyme ZLHY-6. After the zearalenone detoxification enzyme ZLHY-6 mutants H134W and Q45C/A253C with higher thermal stability are subjected to heat treatment at 45 DEG C for 20 minutes, the relative enzyme activities of the zearalenone detoxification enzyme ZLHY-6 mutants H134W and Q45C/A253C are respectively 10 times and 3.1 times of those of a wild type, and certain activity (41.33% and 102.67%) on ZEN is reserved. 本发明公开了一种具有较高热稳定性的玉米赤霉烯酮脱毒酶ZLHY-6，其包括突变体H134W或突变体Q45C/A253C，编码突变体H134W的氨基酸序列如SEQ ID NO.1所示，编码突变体Q45C/A253C的氨基酸序列如SEQ ID NO.2所示。本发明具有较高热稳定性的玉米赤霉烯酮脱毒酶ZLHY-6突变体H134W和Q45C/A25