Porcine circovirus type 3 virus-like particle subunit vaccine as well as preparation method and application thereof

The invention discloses a porcine circovirus type 3 virus-like particle subunit vaccine as well as a preparation method and application thereof. An Escherichia coli expression system is adopted, a no-load plasmid pET28a is modified, a strong promoter T7 is replaced with a weak promoter tac, and a di...

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Hauptverfasser:	TANG XIBIAO, HUANG YING, ZHOU MINGGUANG, JIN JIANYUN, LONG YUNZHI, XU GAOYUAN, LI QIANQIAN, YAN WEIDONG, SONG WENBO, YU DAOBING, YANG LIU, HUANG CHAO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a porcine circovirus type 3 virus-like particle subunit vaccine as well as a preparation method and application thereof. An Escherichia coli expression system is adopted, a no-load plasmid pET28a is modified, a strong promoter T7 is replaced with a weak promoter tac, and a dissolution promoting tag GST is introduced, so that an optimized no-load plasmid pETM is obtained, the transcription efficiency is reduced, recombinant protein is protected from being degraded by extracellular protease, the stability of the recombinant protein is improved, and the recombinant protein can be used for preparing the recombinant protein. Then, the PCV3ORF2 sequence is optimized according to the codon preference of escherichia coli, the synthesized optimized sequence is connected with a no-load plasmid pETM, recombinant plasmid transfects escherichia coli, and inducible expression is performed at low temperature, so that the target protein PCV3Cap is completely expressed in a supernatant, the trouble of