Culture method for improving grease emulsifying capacity of antibacterial lipopeptide produced by bacillus licheniformis and application of culture method

The invention provides a culture method for improving the grease emulsifying capacity of antibacterial lipopeptide, which specifically comprises the following steps: inoculating a bacillus licheniformis colony into a culture medium to complete a culture process which comprises one round of low-temperature culture and two rounds of high-temperature culture, and performing filtration, acid precipitation, alcohol-oil extraction, adsorption chromatography and vacuum concentration on the fermentation liquor to obtain the antibacterial lipopeptide. According to the method for producing the antibacterial lipopeptide by culturing the bacillus licheniformis through double pH values (6.5 and 8.5) and double temperatures (32 DEG C and 38 DEG C), the yield of the antibacterial lipopeptide and the emulsification effect are improved through staged culture. 本发明提出了一种提高抗菌脂肽的乳化油脂能力的培养方法，具体如下：将地衣芽孢杆菌菌落接种至培养基之内完成培养过程，所述培养过程包括一轮低温培养以及二轮高温培养，发酵液经过滤、酸沉、醇油提、吸附层析和真空浓缩后即得抗菌脂肽。本发明通过双pH(6.5和8.5)和双温度(32℃和38℃)培养地衣芽孢杆菌产抗菌脂肽的方法，是分阶段培养以提高抗菌脂