Positioning system and method

The invention provides a positioning system and method. The positioning system comprises a positioning frame, a sound transmission net, a positioning gland, an ultrasonic probe, a distance sensor and a server. The positioning frame is of a square tube structure, the sound transmission net is arranged at one end of the positioning frame, the positioning gland is detachably connected to the end, provided with the sound transmission net, of the positioning frame, and a fixing cavity used for fixing in-vitro tissue is formed between the sound transmission net and the positioning gland; the distance sensor is arranged on the side wall of a sound head shell of the ultrasonic probe, and the distance sensor is connected with the server; the ultrasonic probe is used for acquiring an internal image of the in-vitro tissue fixed in the fixed chamber; the server is used for acquiring an internal image of the in-vitro tissue and carrying out image recognition to acquire an image of a focus; the distance sensor is used for