Real-time fluorescent quantitative detection primer, detection method and kit for transgenic soybean MON87712 strain

The invention discloses a real-time fluorescent quantitative detection primer, a real-time fluorescent quantitative detection method and a real-time fluorescent quantitative detection kit for a transgenic soybean MON87712 strain. The detection primer is shown as follows: F: 5 '-GGCCGCAGGAGAAGTT...

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Bibliographische Detailangaben
Hauptverfasser:	GUAN LIJUN, ZHENG GUANJIN, WEI SHUANG, LIU ERLONG, LI ZHIYONG, LU LI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a real-time fluorescent quantitative detection primer, a real-time fluorescent quantitative detection method and a real-time fluorescent quantitative detection kit for a transgenic soybean MON87712 strain. The detection primer is shown as follows: F: 5 '-GGCCGCAGGAGAAGTTATCA-3', F: 5 '- And R is 5 '-TGACCGAGCTGTCTTGTCCTC-3', and the structural formula is shown in the description. According to the invention, a primer and a TaqMan probe are designed according to a specific sequence of a transgenic soybean MON87712 strain, and a detection method of the real-time fluorescent polymerase chain reaction of the transgenic soybean MON87712 at the annealing temperature of 60 DEG C is established. By utilizing the detection primer, the detection probe and an established real-time fluorescent PCR system, the lower limit of quantification of the method is 40 copies, the standard deviation and the relative standard deviation of the MON87712 detection method disclosed by the invention are both within