RT-qPCR (real-time quantitative polymerase chain reaction) analysis and visual transformation method thereof

The invention discloses an RT-qPCR (real-time quantitative polymerase chain reaction) analysis and visual conversion method, which comprises the following steps of: calculating and processing a data result of a real-time fluorescent quantitative PCR technology, converting the data result into a gray-scale image for outputting, and converting a developing image of a protein immunoblotting technology into the gray-scale image for outputting. The method has the beneficial effects that the calculation efficiency is high, the calculation result is accurate, the design is ingenious, and compared with the prior art, the expression quantity change of mRNA under different treatment samples and the correlation strength between mRNA and protein can be very intuitively seen, so that the scientific research efficiency is greatly improved. 本发明公开一种RT-qPCR分析及其可视化转化的方法，其方法为将实时荧光定量PCR技术的数据结果计算处理后转换为灰阶图像输出，并将蛋白质免疫印迹技术的显影成像转换为灰阶图像输出。有益效果：计算效率高，计算结果精准，设计巧妙，与现有技术相比，能非常直观地看到mRNA在不同处理样本下的表达量变化，以及mRNA与蛋白之间的相关性强弱，大大提高了科研效率。