Method for optimizing fermentation conditions for producing (+) gamma-lactamase

The invention belongs to the technical field of bioengineering, and discloses a method for optimizing fermentation conditions of a strain for producing (+) gamma-lactamase. The optimization method of the fermentation conditions of the (+) gamma-lactamase producing strain is as follows: the initial temperature of a culture medium is 25.0 DEG C, the pH value is 7.6 and the mass concentration of glucose is 4.0 g/L. The method comprises the following steps: firstly, screening three factors with large influence, namely, the initial mass concentration of glucose, the p H value and the temperature by using a Plackett-Burman method, and then carrying out a steepest climbing experiment to gradually change the initial pH value, the culture temperature and the mass concentration of glucose, so as to obtain the fermentation conditions of the (+) gamma-lactamase producing strain. And finally, carrying out regression analysis by using Box-Benhnken and utilizing Design-Expert.V8.6. 0.1 software, so as to obtain the optimal