Mixed epithelial mesenchymal phenotype tumor cell model based on Keratin 5 bidirectional regulation and control function and construction method of mixed epithelial mesenchymal phenotype tumor cell model
The invention discloses a mixed epithelial mesenchymal phenotype tumor cell model based on a keratin5 bidirectional regulation function, belongs to the technical field of tumor models, the model is a stable mixed E/M phenotype cell model obtained after keratin5 gene knock-down is carried out on epit...
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| creator | CHEN DAN GU CUIRONG LI DONGXU LIU HUIFEN CAO BANGRONG MI KUN |
| description | The invention discloses a mixed epithelial mesenchymal phenotype tumor cell model based on a keratin5 bidirectional regulation function, belongs to the technical field of tumor models, the model is a stable mixed E/M phenotype cell model obtained after keratin5 gene knock-down is carried out on epithelial cells, and the invention further discloses a construction method of the model. The mixed E/M phenotype cell stemness, the in-vitro migration and invasion ability and the drug resistance to the chemotherapeutic drug cis-platinum of the cell model are all remarkably enhanced, so that convenience can be provided for research on tumor invasion and metastasis.
本发明公开了一种基于keratin5双向调控功能的混合上皮间充质表型肿瘤细胞模型，属于肿瘤模型技术领域，所述模型为对上皮型细胞进行keratin5的基因敲降后获得的稳定的混合E/M表型细胞模型，本发明还公开了上述模型的构建方法；本发明的细胞模型的混合E/M表型细胞干性、体外迁移及侵袭能力、以及对化疗药物顺铂的耐药性均显著增强，从而能够对肿瘤侵袭转移研究提供便利。 |
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本发明公开了一种基于keratin5双向调控功能的混合上皮间充质表型肿瘤细胞模型，属于肿瘤模型技术领域，所述模型为对上皮型细胞进行keratin5的基因敲降后获得的稳定的混合E/M表型细胞模型，本发明还公开了上述模型的构建方法；本发明的细胞模型的混合E/M表型细胞干性、体外迁移及侵袭能力、以及对化疗药物顺铂的耐药性均显著增强，从而能够对肿瘤侵袭转移研究提供便利。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230418&DB=EPODOC&CC=CN&NR=115975948A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230418&DB=EPODOC&CC=CN&NR=115975948A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>CHEN DAN</creatorcontrib><creatorcontrib>GU CUIRONG</creatorcontrib><creatorcontrib>LI DONGXU</creatorcontrib><creatorcontrib>LIU HUIFEN</creatorcontrib><creatorcontrib>CAO BANGRONG</creatorcontrib><creatorcontrib>MI KUN</creatorcontrib><title>Mixed epithelial mesenchymal phenotype tumor cell model based on Keratin 5 bidirectional regulation and control function and construction method of mixed epithelial mesenchymal phenotype tumor cell model</title><description>The invention discloses a mixed epithelial mesenchymal phenotype tumor cell model based on a keratin5 bidirectional regulation function, belongs to the technical field of tumor models, the model is a stable mixed E/M phenotype cell model obtained after keratin5 gene knock-down is carried out on epithelial cells, and the invention further discloses a construction method of the model. The mixed E/M phenotype cell stemness, the in-vitro migration and invasion ability and the drug resistance to the chemotherapeutic drug cis-platinum of the cell model are all remarkably enhanced, so that convenience can be provided for research on tumor invasion and metastasis.
本发明公开了一种基于keratin5双向调控功能的混合上皮间充质表型肿瘤细胞模型，属于肿瘤模型技术领域，所述模型为对上皮型细胞进行keratin5的基因敲降后获得的稳定的混合E/M表型细胞模型，本发明还公开了上述模型的构建方法；本发明的细胞模型的混合E/M表型细胞干性、体外迁移及侵袭能力、以及对化疗药物顺铂的耐药性均显著增强，从而能够对肿瘤侵袭转移研究提供便利。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqdTktqwzAU9CaLkvQOrwfIwqSmzbKYhEBIV90bRR7HAuk9IT1DfMZcqirtosuQ1XyYGeapup3cFT0hOh3hnfEUkMF2nEPhcQSLzhGkU5BEFr4EpIens8mlJ0xHJKOOqaGz612CVSdcugmXyZsfQYZ7ssKaxNMwsf1vZk3TrxGgo5TJgcJjn1bVYjA-4_kPl9XLfvfVHtaI0iFHY8HQrv2s62b71mxf3z8292S-AUhnZtg</recordid><startdate>20230418</startdate><enddate>20230418</enddate><creator>CHEN DAN</creator><creator>GU CUIRONG</creator><creator>LI DONGXU</creator><creator>LIU HUIFEN</creator><creator>CAO BANGRONG</creator><creator>MI KUN</creator><scope>EVB</scope></search><sort><creationdate>20230418</creationdate><title>Mixed epithelial mesenchymal phenotype tumor cell model based on Keratin 5 bidirectional regulation and control function and construction method of mixed epithelial mesenchymal phenotype tumor cell model</title><author>CHEN DAN ; GU CUIRONG ; LI DONGXU ; LIU HUIFEN ; CAO BANGRONG ; MI KUN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115975948A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>CHEN DAN</creatorcontrib><creatorcontrib>GU CUIRONG</creatorcontrib><creatorcontrib>LI DONGXU</creatorcontrib><creatorcontrib>LIU HUIFEN</creatorcontrib><creatorcontrib>CAO BANGRONG</creatorcontrib><creatorcontrib>MI KUN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>CHEN DAN</au><au>GU CUIRONG</au><au>LI DONGXU</au><au>LIU HUIFEN</au><au>CAO BANGRONG</au><au>MI KUN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Mixed epithelial mesenchymal phenotype tumor cell model based on Keratin 5 bidirectional regulation and control function and construction method of mixed epithelial mesenchymal phenotype tumor cell model</title><date>2023-04-18</date><risdate>2023</risdate><abstract>The invention discloses a mixed epithelial mesenchymal phenotype tumor cell model based on a keratin5 bidirectional regulation function, belongs to the technical field of tumor models, the model is a stable mixed E/M phenotype cell model obtained after keratin5 gene knock-down is carried out on epithelial cells, and the invention further discloses a construction method of the model. The mixed E/M phenotype cell stemness, the in-vitro migration and invasion ability and the drug resistance to the chemotherapeutic drug cis-platinum of the cell model are all remarkably enhanced, so that convenience can be provided for research on tumor invasion and metastasis.
本发明公开了一种基于keratin5双向调控功能的混合上皮间充质表型肿瘤细胞模型，属于肿瘤模型技术领域，所述模型为对上皮型细胞进行keratin5的基因敲降后获得的稳定的混合E/M表型细胞模型，本发明还公开了上述模型的构建方法；本发明的细胞模型的混合E/M表型细胞干性、体外迁移及侵袭能力、以及对化疗药物顺铂的耐药性均显著增强，从而能够对肿瘤侵袭转移研究提供便利。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Mixed epithelial mesenchymal phenotype tumor cell model based on Keratin 5 bidirectional regulation and control function and construction method of mixed epithelial mesenchymal phenotype tumor cell model |
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