Mixed epithelial mesenchymal phenotype tumor cell model based on Keratin 5 bidirectional regulation and control function and construction method of mixed epithelial mesenchymal phenotype tumor cell model

The invention discloses a mixed epithelial mesenchymal phenotype tumor cell model based on a keratin5 bidirectional regulation function, belongs to the technical field of tumor models, the model is a stable mixed E/M phenotype cell model obtained after keratin5 gene knock-down is carried out on epithelial cells, and the invention further discloses a construction method of the model. The mixed E/M phenotype cell stemness, the in-vitro migration and invasion ability and the drug resistance to the chemotherapeutic drug cis-platinum of the cell model are all remarkably enhanced, so that convenience can be provided for research on tumor invasion and metastasis. 本发明公开了一种基于keratin5双向调控功能的混合上皮间充质表型肿瘤细胞模型，属于肿瘤模型技术领域，所述模型为对上皮型细胞进行keratin5的基因敲降后获得的稳定的混合E/M表型细胞模型，本发明还公开了上述模型的构建方法；本发明的细胞模型的混合E/M表型细胞干性、体外迁移及侵袭能力、以及对化疗药物顺铂的耐药性均显著增强，从而能够对肿瘤侵袭转移研究提供便利。