Single-stranded circular DNA virus GyH1 recombinant multi-epitope adenovirus vector vaccine and application thereof

The invention discloses a single-stranded circular DNA virus GyH1 recombinant multi-epitope adenovirus vector vaccine and application thereof, and belongs to the technical field of vaccines. The method comprises the following steps: screening antigen epitopes of GyH1VP1, VP2 and VP3 proteins through...

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Bibliographische Detailangaben
Hauptverfasser:	SHI HENGYANG, YANG QI, YAN TIANXING, LANG FENG, YANG MENGZAN, ZHANG LI, CHENG ZIQIANG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a single-stranded circular DNA virus GyH1 recombinant multi-epitope adenovirus vector vaccine and application thereof, and belongs to the technical field of vaccines. The method comprises the following steps: screening antigen epitopes of GyH1VP1, VP2 and VP3 proteins through an online biological information analysis server, connecting the antigen epitopes by using Linker to construct a multi-epitope vaccine GyMEV, and carrying out reverse translation and codon optimization to obtain a nucleotide sequence of the GyMEV. The preparation method comprises the following steps: cloning an antigen polypeptide GyMEV nucleotide sequence into a plasmid vector pMD19-T through artificial synthesis, directionally cloning the antigen polypeptide GyMEV nucleotide sequence into a linearized adenovirus vector by utilizing a seamless cloning technology to obtain a recombinant adenovirus plasmid rAd5-GyMEV, and transfecting the recombinant plasmid into an HEK 293 cell to obtain the recombinant multi-epit