Method for reducing browning of kidney bean in-vitro regeneration system
The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium,...
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| creator | TAN LIANHONG ZHANG HUINA MA LIUHUI MO LONGPING DAI FEI LI YIQING YU JIANGMIN SU SHAOYANG WEI YUAN WU XIAOLI |
| description | The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium, and inducing to form aseptic seedlings; cutting cotyledonary nodes from small hearts of the obtained aseptic seedlings by using a sterilized scalpel, inoculating the cotyledonary nodes into an anti-browning culture medium, and culturing for 1 week; and then cutting off main axillary buds, and transferring the bottle into an anti-browning culture medium for subculture for 2 weeks. Wherein the anti-browning culture medium consists of a basic culture medium and an allogenic material. According to the method, aseptic seedlings are formed through pretreatment induction, then cotyledonary nodes are inoculated into an anti-browning culture medium for primary culture and subculture, the browning phenomenon of kidney bean |
| format | Patent |
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Wherein the anti-browning culture medium consists of a basic culture medium and an allogenic material. According to the method, aseptic seedlings are formed through pretreatment induction, then cotyledonary nodes are inoculated into an anti-browning culture medium for primary culture and subculture, the browning phenomenon of kidney bean</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230418&DB=EPODOC&CC=CN&NR=115968787A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76516</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230418&DB=EPODOC&CC=CN&NR=115968787A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TAN LIANHONG</creatorcontrib><creatorcontrib>ZHANG HUINA</creatorcontrib><creatorcontrib>MA LIUHUI</creatorcontrib><creatorcontrib>MO LONGPING</creatorcontrib><creatorcontrib>DAI FEI</creatorcontrib><creatorcontrib>LI YIQING</creatorcontrib><creatorcontrib>YU JIANGMIN</creatorcontrib><creatorcontrib>SU SHAOYANG</creatorcontrib><creatorcontrib>WEI YUAN</creatorcontrib><creatorcontrib>WU XIAOLI</creatorcontrib><title>Method for reducing browning of kidney bean in-vitro regeneration system</title><description>The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium, and inducing to form aseptic seedlings; cutting cotyledonary nodes from small hearts of the obtained aseptic seedlings by using a sterilized scalpel, inoculating the cotyledonary nodes into an anti-browning culture medium, and culturing for 1 week; and then cutting off main axillary buds, and transferring the bottle into an anti-browning culture medium for subculture for 2 weeks. Wherein the anti-browning culture medium consists of a basic culture medium and an allogenic material. 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Wherein the anti-browning culture medium consists of a basic culture medium and an allogenic material. According to the method, aseptic seedlings are formed through pretreatment induction, then cotyledonary nodes are inoculated into an anti-browning culture medium for primary culture and subculture, the browning phenomenon of kidney bean</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Method for reducing browning of kidney bean in-vitro regeneration system |
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