Method for reducing browning of kidney bean in-vitro regeneration system

The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium,...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: TAN LIANHONG, ZHANG HUINA, MA LIUHUI, MO LONGPING, DAI FEI, LI YIQING, YU JIANGMIN, SU SHAOYANG, WEI YUAN, WU XIAOLI
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium, and inducing to form aseptic seedlings; cutting cotyledonary nodes from small hearts of the obtained aseptic seedlings by using a sterilized scalpel, inoculating the cotyledonary nodes into an anti-browning culture medium, and culturing for 1 week; and then cutting off main axillary buds, and transferring the bottle into an anti-browning culture medium for subculture for 2 weeks. Wherein the anti-browning culture medium consists of a basic culture medium and an allogenic material. According to the method, aseptic seedlings are formed through pretreatment induction, then cotyledonary nodes are inoculated into an anti-browning culture medium for primary culture and subculture, the browning phenomenon of kidney bean