Method for reducing browning of kidney bean in-vitro regeneration system
The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium,...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for reducing browning of a kidney bean in-vitro regeneration system, which comprises primary culture and rooting culture, the primary culture comprises the following steps: selecting full and fresh seeds, pretreating, inoculating into an MS-containing culture medium, and inducing to form aseptic seedlings; cutting cotyledonary nodes from small hearts of the obtained aseptic seedlings by using a sterilized scalpel, inoculating the cotyledonary nodes into an anti-browning culture medium, and culturing for 1 week; and then cutting off main axillary buds, and transferring the bottle into an anti-browning culture medium for subculture for 2 weeks. Wherein the anti-browning culture medium consists of a basic culture medium and an allogenic material. According to the method, aseptic seedlings are formed through pretreatment induction, then cotyledonary nodes are inoculated into an anti-browning culture medium for primary culture and subculture, the browning phenomenon of kidney bean |
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