Establishment method of Chinese cherry regeneration system
The invention discloses a method for establishing a Chinese cherry regeneration system, which comprises the following steps: (1) material pretreatment: breaking seed shells of Chinese cherry kernels, and taking out complete seed embryos; (2) sterilization: soaking the embryos with 75% alcohol and 0....
Gespeichert in:
| Hauptverfasser: | , , , , , , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | TU HONGXIA ZHANG BO LUO XIRUI WANG YI LIU ZHENSHAN WANG XIAORONG WANG HAO CHEN QING WANG YAN ZHANG JING |
| description | The invention discloses a method for establishing a Chinese cherry regeneration system, which comprises the following steps: (1) material pretreatment: breaking seed shells of Chinese cherry kernels, and taking out complete seed embryos; (2) sterilization: soaking the embryos with 75% alcohol and 0.1% mercury bichloride in sequence for sterilization; (3) aseptic seedling culture: inoculating the sterilized embryos on a culture medium, and performing dark culture to germinate seedlings; (4) adventitious bud induction: cutting off cotyledons when the seedlings grow to a two-leaf and one-core state, and inoculating the cotyledons into an adventitious bud induction culture medium; (5) rooting culture: when the culture material grows to 2-3cm, transferring the culture material to a rooting culture medium to obtain tissue culture seedlings; and (6) transplanting: transplanting the tissue culture seedlings into a matrix for culture. According to the method, cotyledons 30-36 days after flowering of the Chinese cherri |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN115968785A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN115968785A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN115968785A3</originalsourceid><addsrcrecordid>eNrjZLByLS5JTMrJLM7ITc0rUchNLcnIT1HIT1NwzsjMSy1OVUjOSC0qqlQoSk1PzUstSizJzM9TKK4sLknN5WFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFiclAHSXxzn6GhqaWZhbmFqaOxsSoAQCq_C-i</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>Establishment method of Chinese cherry regeneration system</title><source>esp@cenet</source><creator>TU HONGXIA ; ZHANG BO ; LUO XIRUI ; WANG YI ; LIU ZHENSHAN ; WANG XIAORONG ; WANG HAO ; CHEN QING ; WANG YAN ; ZHANG JING</creator><creatorcontrib>TU HONGXIA ; ZHANG BO ; LUO XIRUI ; WANG YI ; LIU ZHENSHAN ; WANG XIAORONG ; WANG HAO ; CHEN QING ; WANG YAN ; ZHANG JING</creatorcontrib><description>The invention discloses a method for establishing a Chinese cherry regeneration system, which comprises the following steps: (1) material pretreatment: breaking seed shells of Chinese cherry kernels, and taking out complete seed embryos; (2) sterilization: soaking the embryos with 75% alcohol and 0.1% mercury bichloride in sequence for sterilization; (3) aseptic seedling culture: inoculating the sterilized embryos on a culture medium, and performing dark culture to germinate seedlings; (4) adventitious bud induction: cutting off cotyledons when the seedlings grow to a two-leaf and one-core state, and inoculating the cotyledons into an adventitious bud induction culture medium; (5) rooting culture: when the culture material grows to 2-3cm, transferring the culture material to a rooting culture medium to obtain tissue culture seedlings; and (6) transplanting: transplanting the tissue culture seedlings into a matrix for culture. According to the method, cotyledons 30-36 days after flowering of the Chinese cherri</description><language>chi ; eng</language><subject>AGRICULTURE ; ANIMAL HUSBANDRY ; FISHING ; FORESTRY ; HUMAN NECESSITIES ; HUNTING ; NEW PLANTS OR PROCESSES FOR OBTAINING THEM ; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES ; TRAPPING</subject><creationdate>2023</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230418&DB=EPODOC&CC=CN&NR=115968785A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76290</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20230418&DB=EPODOC&CC=CN&NR=115968785A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>TU HONGXIA</creatorcontrib><creatorcontrib>ZHANG BO</creatorcontrib><creatorcontrib>LUO XIRUI</creatorcontrib><creatorcontrib>WANG YI</creatorcontrib><creatorcontrib>LIU ZHENSHAN</creatorcontrib><creatorcontrib>WANG XIAORONG</creatorcontrib><creatorcontrib>WANG HAO</creatorcontrib><creatorcontrib>CHEN QING</creatorcontrib><creatorcontrib>WANG YAN</creatorcontrib><creatorcontrib>ZHANG JING</creatorcontrib><title>Establishment method of Chinese cherry regeneration system</title><description>The invention discloses a method for establishing a Chinese cherry regeneration system, which comprises the following steps: (1) material pretreatment: breaking seed shells of Chinese cherry kernels, and taking out complete seed embryos; (2) sterilization: soaking the embryos with 75% alcohol and 0.1% mercury bichloride in sequence for sterilization; (3) aseptic seedling culture: inoculating the sterilized embryos on a culture medium, and performing dark culture to germinate seedlings; (4) adventitious bud induction: cutting off cotyledons when the seedlings grow to a two-leaf and one-core state, and inoculating the cotyledons into an adventitious bud induction culture medium; (5) rooting culture: when the culture material grows to 2-3cm, transferring the culture material to a rooting culture medium to obtain tissue culture seedlings; and (6) transplanting: transplanting the tissue culture seedlings into a matrix for culture. According to the method, cotyledons 30-36 days after flowering of the Chinese cherri</description><subject>AGRICULTURE</subject><subject>ANIMAL HUSBANDRY</subject><subject>FISHING</subject><subject>FORESTRY</subject><subject>HUMAN NECESSITIES</subject><subject>HUNTING</subject><subject>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</subject><subject>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</subject><subject>TRAPPING</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2023</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZLByLS5JTMrJLM7ITc0rUchNLcnIT1HIT1NwzsjMSy1OVUjOSC0qqlQoSk1PzUstSizJzM9TKK4sLknN5WFgTUvMKU7lhdLcDIpuriHOHrqpBfnxqcUFiclAHSXxzn6GhqaWZhbmFqaOxsSoAQCq_C-i</recordid><startdate>20230418</startdate><enddate>20230418</enddate><creator>TU HONGXIA</creator><creator>ZHANG BO</creator><creator>LUO XIRUI</creator><creator>WANG YI</creator><creator>LIU ZHENSHAN</creator><creator>WANG XIAORONG</creator><creator>WANG HAO</creator><creator>CHEN QING</creator><creator>WANG YAN</creator><creator>ZHANG JING</creator><scope>EVB</scope></search><sort><creationdate>20230418</creationdate><title>Establishment method of Chinese cherry regeneration system</title><author>TU HONGXIA ; ZHANG BO ; LUO XIRUI ; WANG YI ; LIU ZHENSHAN ; WANG XIAORONG ; WANG HAO ; CHEN QING ; WANG YAN ; ZHANG JING</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN115968785A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2023</creationdate><topic>AGRICULTURE</topic><topic>ANIMAL HUSBANDRY</topic><topic>FISHING</topic><topic>FORESTRY</topic><topic>HUMAN NECESSITIES</topic><topic>HUNTING</topic><topic>NEW PLANTS OR PROCESSES FOR OBTAINING THEM</topic><topic>PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES</topic><topic>TRAPPING</topic><toplevel>online_resources</toplevel><creatorcontrib>TU HONGXIA</creatorcontrib><creatorcontrib>ZHANG BO</creatorcontrib><creatorcontrib>LUO XIRUI</creatorcontrib><creatorcontrib>WANG YI</creatorcontrib><creatorcontrib>LIU ZHENSHAN</creatorcontrib><creatorcontrib>WANG XIAORONG</creatorcontrib><creatorcontrib>WANG HAO</creatorcontrib><creatorcontrib>CHEN QING</creatorcontrib><creatorcontrib>WANG YAN</creatorcontrib><creatorcontrib>ZHANG JING</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>TU HONGXIA</au><au>ZHANG BO</au><au>LUO XIRUI</au><au>WANG YI</au><au>LIU ZHENSHAN</au><au>WANG XIAORONG</au><au>WANG HAO</au><au>CHEN QING</au><au>WANG YAN</au><au>ZHANG JING</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Establishment method of Chinese cherry regeneration system</title><date>2023-04-18</date><risdate>2023</risdate><abstract>The invention discloses a method for establishing a Chinese cherry regeneration system, which comprises the following steps: (1) material pretreatment: breaking seed shells of Chinese cherry kernels, and taking out complete seed embryos; (2) sterilization: soaking the embryos with 75% alcohol and 0.1% mercury bichloride in sequence for sterilization; (3) aseptic seedling culture: inoculating the sterilized embryos on a culture medium, and performing dark culture to germinate seedlings; (4) adventitious bud induction: cutting off cotyledons when the seedlings grow to a two-leaf and one-core state, and inoculating the cotyledons into an adventitious bud induction culture medium; (5) rooting culture: when the culture material grows to 2-3cm, transferring the culture material to a rooting culture medium to obtain tissue culture seedlings; and (6) transplanting: transplanting the tissue culture seedlings into a matrix for culture. According to the method, cotyledons 30-36 days after flowering of the Chinese cherri</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN115968785A |
| source | esp@cenet |
| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | Establishment method of Chinese cherry regeneration system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T04%3A39%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=TU%20HONGXIA&rft.date=2023-04-18&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN115968785A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |