Establishment method of Chinese cherry regeneration system

The invention discloses a method for establishing a Chinese cherry regeneration system, which comprises the following steps: (1) material pretreatment: breaking seed shells of Chinese cherry kernels, and taking out complete seed embryos; (2) sterilization: soaking the embryos with 75% alcohol and 0.1% mercury bichloride in sequence for sterilization; (3) aseptic seedling culture: inoculating the sterilized embryos on a culture medium, and performing dark culture to germinate seedlings; (4) adventitious bud induction: cutting off cotyledons when the seedlings grow to a two-leaf and one-core state, and inoculating the cotyledons into an adventitious bud induction culture medium; (5) rooting culture: when the culture material grows to 2-3cm, transferring the culture material to a rooting culture medium to obtain tissue culture seedlings; and (6) transplanting: transplanting the tissue culture seedlings into a matrix for culture. According to the method, cotyledons 30-36 days after flowering of the Chinese cherri