Tissue culture and rapid propagation method for tender shoots of radix asparagi in southwest

The invention discloses a method for tissue culture and rapid propagation of tender shoots of southwest asparagus cochinchinensis, and relates to the field of tissue culture of medicinal plants, new tender shoot branches of the southwest asparagus cochinchinensis of two years old or more are used as explants, and the method comprises the following steps of plant disinfection, callus induction, cluster bud induction, rooting induction and seedling hardening domestication. In the seedling hardening and domestication step, seedlings are soaked in a root promoting solution to promote rooting of the seedlings, then the seedlings are cultivated on a seedbed prepared from peat soil, humus soil and river sand according to the proportion of 2: 1: 1, and the southwest asparagus cochinchinensis seedlings are obtained through a tissue culture method. According to the method, the induction rate of each stage of tissue culture is high, the survival rate of the seedlings is remarkable, the tissue culture period is short, th