Newcastle disease hemagglutination inhibition antigen and preparation method thereof

The invention provides a Newcastle disease hemagglutination inhibition antigen and a preparation method thereof. The preparation method comprises the following steps: inoculating an SPF chick embryo with a Newcastle disease La Sota strain, culturing, harvesting a culture, concentrating, centrifuging and inactivating the culture, mixing with a heat-resistant protective agent, and freeze-drying to prepare the Newcastle disease hemagglutination inhibition antigen. The solid Newcastle disease hemagglutination inhibition antigen is prepared by concentrating, centrifuging and inactivating the culture, mixing with the heat-resistant protective agent and freeze-drying, the antigen protection time is long, the difference between batches is small, and the key problem of the Newcastle disease detection antigen preparation process is solved; and a good detection antigen is provided for the detection of the Newcastle disease antibody in the reproduction and inspection process of veterinary biological product enterprises.