Silver nano-cluster in-situ synthesis method based on catalytic hairpin self-assembly

The invention discloses a silver nano-cluster in-situ synthesis method based on catalytic hairpin self-assembly, which is characterized in that a functional hairpin H1 is designed, a target object can be recognized to form hybrid double strands, a synthesis template of a silver nano-cluster is designed at the tail end of the hairpin H1, Ag < + > is tightly combined with a template fragment, Ag < + > is reduced into Ag under the reduction action of NaBH4, and the silver nano-cluster is obtained. A fluorescent silver nano-cluster is formed through a certain accumulation effect, and a target object is replaced from a hybrid double strand by a hairpin H2, so that target object amplification circulation and real-time quantitative label-free detection of a fluorescent signal are realized. 本专利公开一种基于催化发夹自组装的银纳米簇原位合成方法，该方法设计了一种功能发夹H1，能够识别目标物形成杂交双链以及在发夹H1末端设计了银纳米簇的合成模板，Ag+与模板片段紧密结合，在NaBH4的还原作用下，Ag+被还原成Ag，通过一定的堆积作用形成荧光银纳米簇，发夹H2将目标物从杂交双链上置换下来，实现目标物扩增循环与荧光信号实时定量的无标记检测。