Method for separating and purifying (-) gamma-lactam

The invention belongs to the technical field of bioengineering, and discloses a method for separating and purifying (-) gamma-lactam. By regulating and controlling physical and chemical factors in a reaction system, (+) gamma-lactam in (+/-) gamma-lactam is degraded with high stereoselectivity by ut...

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Hauptverfasser: WANG CONG, LIU CHUNYING, CHI XUEMEI, XIA SHUIYING, CHI NAIYU, YU SHUANG, GUO XINZHI, YUAN WENTAO, ZHANG QINGFANG, WANG YAN
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creator WANG CONG
LIU CHUNYING
CHI XUEMEI
XIA SHUIYING
CHI NAIYU
YU SHUANG
GUO XINZHI
YUAN WENTAO
ZHANG QINGFANG
WANG YAN
description The invention belongs to the technical field of bioengineering, and discloses a method for separating and purifying (-) gamma-lactam. By regulating and controlling physical and chemical factors in a reaction system, (+) gamma-lactam in (+/-) gamma-lactam is degraded with high stereoselectivity by utilizing the screened strain for producing (+) gamma-lactamase to obtain (-) gamma-lactam, and then the (-) gamma-lactam is separated and purified so as to meet the requirements of industrial production. 本发明属于生物工程技术领域,公开了一种分离提纯(-)γ-内酰胺的方法。通过调控反应体系中的理化因素,利用筛选得到的产(+)γ-内酰胺酶菌株高立体选择性降解(±)γ-内酰胺中的(+)γ-内酰胺,得到(-)γ-内酰胺,进而对(-)γ-内酰胺进行分离提纯,以期来满足工业生产的需要。
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
title Method for separating and purifying (-) gamma-lactam
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