Entomopathogenic nematode symbiotic bacterium fermentation supernatant preparation, preparation method and application

The invention belongs to the technical field of biology, and particularly relates to an entomopathogenic nematode symbiotic bacterium fermentation supernatant preparation which comprises enteric nematode lyophilus LY2W fermentation supernatant and an auxiliary. The preparation method comprises the f...

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Hauptverfasser: YIN SHOUHENG, MA JINHAI, WANG LIANZHE, WANG YANBIN, LIAO CHUNLI, MA WEIHAO, ZHAO YIKAI, JI XIAONA, MA DONGHAO, LI JIJUN, LI BINGBING, FU TAOTAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of biology, and particularly relates to an entomopathogenic nematode symbiotic bacterium fermentation supernatant preparation which comprises enteric nematode lyophilus LY2W fermentation supernatant and an auxiliary. The preparation method comprises the following steps: S1, selecting single colonies on an NBTA identification culture medium; s2, picking single colonies on the NA culture medium; s3, culturing with an LB liquid culture medium; s4, shake-flask fermentation culture; s5, centrifuging to obtain supernate; and S6, uniformly mixing the supernatant with an auxiliary agent to obtain the entomopathogenic nematode symbiotic bacterium fermentation supernatant preparation. The invention further provides application of the entomopathogenic nematode symbiotic bacterium fermentation supernatant preparation in prevention and treatment of leek gray mold and tomato gray mold. The entomopathogenic nematode symbiotic bacterium fermentation supernatant preparation disclos