Kit for immunofluorescence detection, use method and fluorescence immunodetection device

The invention relates to a kit for immunofluorescence detection, which comprises a kit body, and the kit body comprises a storage chamber and a transfer cavity; external force is applied to the transfer cavity, so that negative pressure is formed in the transfer cavity by increasing the volume of air in the transfer cavity, and the trace reagent in the storage chamber flows into the transfer cavity; and/or external force is applied to the transfer cavity, so that positive pressure is formed in the transfer cavity by reducing the volume of air in the transfer cavity, and the trace reagent in the transfer cavity is sucked back to the storage chamber. The invention also relates to a method for transferring liquid by changing the volume of the transfer cavity, a use method of the kit and a fluorescence immunoassay device. By operating the power device, liquid is transferred in a closed environment, the required sample size is small, the operation is simple and convenient, and transfer can be completed in a short