Engineering frozen liver cancer cell carrier as well as preparation method and application thereof

The invention provides a preparation method of an engineered frozen liver cancer cell carrier, the cell carrier obtained according to the preparation method and application of the cell carrier in the process of relieving atherosclerosis. The method comprises the following steps: stimulating hepatoma carcinoma cells cultured in vitro to express apolipoprotein ApoA1 by using bafluromycin A1; performing liquid nitrogen freezing on the treated live liver cancer cells to obtain frozen liver cancer cells; and incubating the frozen liver cancer cells and atorvastatin (AT) to obtain the frozen liver cancer cell carrier. The carrier completely loads a drug atorvastatin (AT). The frozen liver cancer cell carrier can effectively inhibit autophagy of liver cancer cells after being treated by the bajulomycin A1, so that expression of apolipoprotein (ApoA1) is promoted, and the ApoA1 and phospholipid can form beta-HDL to uptake cholesterol and transfer the cholesterol to the liver for metabolism; meanwhile, after the carri