Quantum dot fluorescent microsphere labeled double-flux immunochromatography detection card and application thereof

The invention discloses a quantum dot fluorescent microsphere labeled double-flux immunochromatography detection card and application thereof. The double-flux immunochromatography test strip comprises a PVC (polyvinyl chloride) back plate as well as a sample pad, a combination pad, a reaction pad and an absorption pad which are sequentially arranged on the PVC back plate, and adjacent pads need to be partially overlapped; a detection line T2 line, a detection line T1 line and a quality control line C line which are parallel to one another are sequentially arranged on the reaction pad from the end close to the combination pad to the end close to the absorption pad, the detection line T1 line and the detection line T2 line are respectively coated with recombinant GP5 protein and recombinant N protein, and the quality control line is coated with a goat anti-mouse IgG antibody; and the combination pad contains mouse anti-pig IgG antibody-biotin-avidin compound-quantum dot fluorescent microspheres. The invention f