Prawn Taura syndrome virus rapid detection method based on ERA technology and primer and probe combination

The invention provides a prawn Taura syndrome virus rapid detection method based on an ERA technology and a primer and probe combination. The Taura syndrome virus capsid protein 2 gene is used as a target gene for detection, a special primer and a probe for ERA detection are designed and synthesized aiming at the target gene, and an ERA detection method of the Taura syndrome virus is established. The method disclosed by the invention is simple in operation steps, short in time consumption, high in speed, accurate and reliable, and relatively high in detection sensitivity (the sensitivity can reach 101 copies/mu L), and can meet the requirement of detecting the prawn Taura syndrome virus on site in a farm. 本发明提供一种基于ERA技术的对虾桃拉综合征病毒快速检测方法及引物与探针组合。本发明以桃拉综合征病毒衣壳蛋白2基因作为检测的靶基因，针对靶基因设计合成ERA检测专用引物和探针，并建立桃拉综合征病毒的ERA检测方法。本发明方法操作步骤简单，耗时短，速度快，准确可靠，检测灵敏度较高(灵敏度可达101copies/μL)，可满足养殖场现场检测对虾桃拉综合征病毒的需求。