Primer group and method for detecting non-deletion type thalassemia and application of primer group and method

The invention discloses a primer group for detecting non-deletion type thalassemia. The sequence of the primer group comprises a multiple amplification primer sequence as shown in SeqID NO.1-22 and an extension primer sequence as shown in SeqID NO.23-46; the multiple amplification primer sequences a...

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Bibliographische Detailangaben
Hauptverfasser: XU YANTING, CHEN XIUXIA, CHEN BIXIAO
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a primer group for detecting non-deletion type thalassemia. The sequence of the primer group comprises a multiple amplification primer sequence as shown in SeqID NO.1-22 and an extension primer sequence as shown in SeqID NO.23-46; the multiple amplification primer sequences are divided into two groups, the first group of amplification primer sequences comprises SeqID NO.1-12 and SeqID NO.19-20, and the second group of amplification primer sequences comprises SeqID NO.3-6 and SeqID NO.11-22; the extension primer group sequence is divided into two groups, the first group of extension primer group sequence comprises SeqID NO.23-34, and the second group of extension primer group sequence comprises SeqID NO.35-46. The method is more in line with the characteristics of people in China, overcomes the defect that few SNP sites are detected at one time in the prior art, has the characteristics of high accuracy, high sensitivity, good repeatability, low cost and short detection period, and has a