Coding microbead for single cell detection and preparation method thereof

The invention belongs to the technical field of molecular biology, and particularly relates to a coding microbead for single cell detection and a preparation method thereof. The coding microbead provided by the invention is composed of a support and a coding tag, the size of the coding microbead is 20-65 [mu] m, and the coding tag is formed by combining five sequences including a universal sequence, a cell tag, a molecular tag, an auxiliary sequence and PolydT; according to the invention, molecular sequences with different labels are connected to the microbeads by adopting a Splint connection method, the cost is low, the operation is simple, the synthesis is rapid, and the production process is stable. The coding microbead provided by the invention is high in sequencing accuracy, overcomes the problems of low connection efficiency and complex operation in a multi-step connection process, and can be applied to single-cell high-throughput sequencing, single-cell transcriptome research, single-cell genome resear