Recombinase-mediated full-freeze-drying isothermal amplification system as well as preparation method and application thereof

The invention provides a recombinase-mediated full-freeze-drying isothermal amplification system and a preparation method and application thereof, and relates to the field of molecular biology detection.The full-freeze-drying isothermal amplification system comprises two parts: the first part is an enzyme system; the second part comprises a primer probe and an activation system; the freeze-drying form of the enzyme system is freeze-dried powder; the freeze-drying form of the primer probe and the activation system is a freeze-drying ball. The enzyme system is prepared into the freeze-dried powder, and the activation system and the primer probe are mixed to prepare the freeze-dried ball, so that the smoothness and homogeneity of the liquid are improved, the problem that the result is unstable due to the fact that the whole system is high in viscosity and difficult to disperse and homogenate in the freeze-drying process is solved, RPA full freeze-drying is realized, the operation of one-step sample loading is ac