Method for improving anaerobic activity of bacillus licheniformis by using nitrate and global anaerobic regulatory factor Fnr

The invention discloses a method for improving anaerobic activity of bacillus licheniformis by using nitrate and a global anaerobic regulatory factor Fnr, and belongs to the field of fermentation engineering. KNO3 is added into a fermentation culture medium, so that the anaerobic fermentation activity of the bacillus licheniformis is improved. Furthermore, through overexpression of the fnr gene, the thallus amount of the bacillus licheniformis is increased by 8% compared with that of a control strain, the KNO3 consumption rate is increased by 23% compared with that of the control strain, and 0.9 g/L of lactic acid is accumulated in 24 h. The anaerobic culture method provided by the invention is simple in process, and lays theoretical and experimental foundations for widening anaerobic fermentation application of bacillus licheniformis. 本发明公开了一种利用硝酸盐和全局厌氧调节因子Fnr提高地衣芽孢杆菌厌氧活性的方法，属于发酵工程领域。本发明通过向发酵培养基中加入KNO3，提高地衣芽孢杆菌厌氧发酵的活性。进一步通过过表达fnr基因，使地衣芽孢杆菌的菌体量较对照菌株提高了8％，KNO3消耗速率较对照菌株提高了23％，乳酸在24h积累了0.9g/L。本发明提供的厌氧培养方法工艺简单，为拓