Vesicular stomatitis virus attenuating method, attenuated virus strain and application

The invention discloses a vesicular stomatitis virus attenuating method, an attenuated virus strain and application, and the method comprises the following steps: carrying out amino acid deletion of different quantities and different positions on an amino acid sequence of a vesicular stomatitis virus coding M protein, and obtaining the vesicular stomatitis attenuated virus with corresponding deletion by utilizing a reverse genetic operating system. The virulence of the vesicular stomatitis virus is reduced in an M gene deletion manner, and a foundation is laid for the VSV to become a safer vaccine vector and an oncolytic virus vector for emerging viral diseases. 本发明公开了一种水泡性口炎病毒的致弱方法及致弱病毒株和应用，包括：对水泡性口炎病毒编码M蛋白的氨基酸序列进行不同数量和不同位置的氨基酸缺失，利用反向遗传操作系统获得具有相应缺失的水泡性口炎致弱病毒。本发明以M基因缺失的方式降低了水泡性口炎病毒的毒力，为VSV成为更加安全的新兴病毒性疾病的疫苗载体和溶瘤病毒载体奠定基础。