Kluyveromyces marxianus haploid yeast as well as construction method and application thereof

The invention belongs to the technical field of bioengineering, and particularly relates to Kluyveromyces marxianus haploid yeast as well as a construction method and application thereof. The Kluyveromyces marxianus haploid yeast is a chassis strain, Kluyveromyces marxianus ATCC 36534 is used as a microbial chassis host, and the Kluyveromyces marxianus haploid yeast is obtained on the basis of constructing a MATalpha3 gene defect strain. The recombinant Ergothioneine is obtained by an induced sporulation isolation method and is successfully applied to the fields of efficient biosynthesis of Ergothioneine, extracellular secretion expression of hyaluronic acid degrading enzyme and the like. The Kluyveromyces marxianus strain lays a foundation for developing Kluyveromyces marxianus into a synthetic biological chassis, and application of Kluyveromyces marxianus strain in the biological manufacturing fields of high-added-value chemicals, industrial enzymes and the like is proved, so that the Kluyveromyces marxianu